ENZYMES AND BLOOD CLOTTING. I. TRYPSIN AS AN ACCESSORY FACTOR *
نویسندگان
چکیده
منابع مشابه
The Action of Crystalline Trypsin and Soybean Trypsin Inhibitor on the Clotting of Blood by Staphylocoagulase *
The mechanism of the coagulation of blood by staphylocoagulase and the relation of this process to physiological clotting present many unsolved problems. Evidence has been advanced for the concept that coagulase reacts with a plasma component (coagulase reacting factor CRF) which is distinct from prothrombin.5'4'1S1 The formation of an intermediate complex has been inferred from the shortening ...
متن کاملEffect of Blood Thrombokinase, as Influenced by Soy Bean Trypsin Inhibitor; Ultracentrifugation, and Accessory Factors
1. Crystallized soy bean trypsin inhibitor, at a concentration of 100 microg./ml., suppressed the production of thrombin from a mixture of prothrombin and blood thrombokinase. The experiment was performed in the presence of 0.011 M oxalate, in order to minimize the possibility of participation by accessory factors which require ionic calcium. The results are in accord with the view that thrombo...
متن کاملActivation of coagulase clotting by trypsin inhibitor.
Egg white trypsin inhibitor activated coagulase clotting when added to a final concentration between 2 and 60 mg/ml. The greatest increase in clotting rate was observed in reaction mixtures containing the lowest concentrations of serum and plasma. Maximal activation was reached with 40 mg of trypsin inhibitor per ml when either serum or plasma was used as the source of coagulase-reacting factor...
متن کاملMilk-clotting Enzymes from Microorganisms.
A total of 230 cultures of fungi and 43 cultures of bacteria, isolated from such sources as soil, butter, and milk, were screened for their milk-clotting activity. The fungi were cultivated on semisolid media, and the bacteria were grown in milk media in shake culture. Phytic acid, added as calcium phytate, was found to stimulate production of the enzyme in most of the bacterial isolates. Prote...
متن کاملThe site of inhibition of blood clotting by soy bean trypsin inhibitor.
a cofactor which accelerates the rate of of the enzyme-substrate complex (2). A trypsin inhibitor, isolated from so Kunitz (3), is also capable of delaying t' tion of blood. Previous studies by six investigators have shown that it inhibi phase of coagulation and has no effect tivity of thrombin (4-10). None of ti of workers, however, was able to ascerta the soy bean trypsin inhibitor (SBTI) wit...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1960
ISSN: 0021-9738
DOI: 10.1172/jci104219